Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Adiponectin promotes endotoxin tolerance in macrophages by inducing IRAK-M expression.

doi: 10.4049/jimmunol.0803694

Figure Lengend Snippet: FIGURE 1. A, Thioglycolate-derived mouse peritoneal macrophages from C57BL/6 mice were stimulated with gAd (10 g/ml) or LPS (1 g/ml) as a positive control, for the time points indicated, and IRAK-M expression was determined by Western blotting. B, Thioglycolate-derived mouse peritoneal macrophages from C57BL/6 mice were stimulated with gAd (10 g/ml) for the time points indicated and IRAK-M expression was determined by real-time RT PCR. C and D, Thioglycolate-derived mouse peritoneal macrophages from C57BL/6 mice were pretreated with gAd (10 g/ml) for different time points and then restimulated with LPS; TNF- (C) and IL-6 (D) were measured in the culture supernatants. E, Thioglycolate-derived mouse peritoneal macro- phages from C57BL/6 mice were stimulated with gAd (10 g/ml) or LPS (1 g/ml) as a positive control for 24 h, and IL-10 secretion in the culture supernatants was measured with ELISA. Results represent the average SEM of three independent experiments: , p 0.05; , p 0.01; and , p 0.001 denote statistically significant difference compared with control cells; and ##, p 0.01 and ###, p 0.001 denote statistically significant difference compared with the earlier time point, as indicated by the brackets.

Article Snippet: Plasma from peripheral blood samples was collected and frozen until used for human adiponectin determination by ELISA (R&D Systems).

Techniques: Derivative Assay, Positive Control, Expressing, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Adiponectin promotes endotoxin tolerance in macrophages by inducing IRAK-M expression.

doi: 10.4049/jimmunol.0803694

Figure Lengend Snippet: FIGURE 2. Thioglycolate-derived mouse peritoneal macrophages from IRAK-M/ and IRAK-M/ mice were pretreated with gAd (1 g/ml) for 24 h and restimulated with LPS (1 g/ml) for 4 and 24 h. TNF- (A) secretion was estimated at 4 h, and IL-6 (B) and KC (C) at 24 h, by ELISA. Results represent the average SEM of three independent experiments; , p 0.05 and , p 0.01 denote statistically significant difference compared with non-adiponectin- pretreated IRAK-M/ cells, and ##, p 0.01 denotes statistically significant difference compared with adiponectin-pretreated IRAK-M/ cells. D, Levels of adiponectin were estimated in the plasma of IRAK-M/ and IRAK-M/ mice with ELISA (four mice in each group).

Article Snippet: Plasma from peripheral blood samples was collected and frozen until used for human adiponectin determination by ELISA (R&D Systems).

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Adiponectin promotes endotoxin tolerance in macrophages by inducing IRAK-M expression.

doi: 10.4049/jimmunol.0803694

Figure Lengend Snippet: FIGURE 4. A, Thioglycolate-derived mouse peritoneal macrophages from C57BL/6 mice were pretreated with wortmannin (1 M) for 1 h and restimulated with adiponectin (10 g/ml) and LPS (1 g/ml), as a positive control, for 12 h. IRAK-M expression levels were determined by Western blotting. Results repre- sent the average SEM of three independent experiments; , p 0.001 de- notes statistically significant difference as compared with non-pretreated cells for the respective stimuli. B, Thioglycolate-derived mouse peritoneal macrophages from Akt1/ and Akt1/ mice were stimulated with adiponectin (10 g/ml), for the time points indicated. IRAK-M expression levels were determined by Western blotting. The figure is representative of three independent experiments. C, Thio- glycolate-derived mouse peritoneal macrophages from Akt1/ and Akt1/

Article Snippet: Plasma from peripheral blood samples was collected and frozen until used for human adiponectin determination by ELISA (R&D Systems).

Techniques: Derivative Assay, Positive Control, Expressing, Western Blot

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Adiponectin promotes endotoxin tolerance in macrophages by inducing IRAK-M expression.

doi: 10.4049/jimmunol.0803694

Figure Lengend Snippet: FIGURE 5. Human peripheral monocytes/macrophages were isolated from seven individuals with high plasma adiponectin (12–16 g/ml) and seven individuals with low plasma adiponectin (2.5–5 g/ml). A, Total RNA was isolated and IRAK-M expression was estimated with real-time RT-PCR. B and C, Whole blood was stimulated with LPS for 6 h, and TNF- (B) and IL-6 (C) were measured in the plasma. Results represent the average SEM; , p 0.001 and , p 0.05 denote statistically significant difference compared with subjects with high plasma adiponectin.

Article Snippet: Plasma from peripheral blood samples was collected and frozen until used for human adiponectin determination by ELISA (R&D Systems).

Techniques: Isolation, Clinical Proteomics, Expressing, Quantitative RT-PCR

Journal: Shock (Augusta, Ga.)

Article Title: Soluble T-Cadherin: A Novel Biomarker for Fluid Resuscitation in Burn Patients—A Single-Center Retrospective Observational Study

doi: 10.1097/SHK.0000000000002728

Figure Lengend Snippet: Serum levels of soluble T-cadherin isoforms and endothelial damage markers in burn patients and healthy controls. A, Levels of 130 kDa, 100 kDa, and 30 kDa soluble T-cadherin in controls, survivors, and nonsurvivors during Phases 1 and 2. B, Levels of PAI-1, syndecan-1, glypican-1, and hyaluronan were similarly compared. * P < 0.05, ** P < 0.01. PAI-1, plasminogen activator inhibitor-1.

Article Snippet: Adiponectin and endothelial damage markers were measured using ELISA kits (Human T-Cadherin Quantikine ELISA Kit, R&D Systems, Minneapolis, MN; Catalog Nos: Adiponectin: DY1065-05, PAI-1: DY1786, Hyaluronan: DY3614-05, Syndecan-1: DY2780, Glypican-1: DY4519-05) according to the manufacturer’s instructions.

Techniques:

Journal: Shock (Augusta, Ga.)

Article Title: Soluble T-Cadherin: A Novel Biomarker for Fluid Resuscitation in Burn Patients—A Single-Center Retrospective Observational Study

doi: 10.1097/SHK.0000000000002728

Figure Lengend Snippet: Time-dependent changes in serum adiponectin, T-cadherin, and hematocrit levels within 24 hours postinjury. A, Scatterplot showing the correlation between time from injury to blood sampling and each parameter. B, Subanalysis limited to patients without fluid administration before blood sampling. C, Subanalysis limited to patients with fluid administration before blood sampling.

Article Snippet: Adiponectin and endothelial damage markers were measured using ELISA kits (Human T-Cadherin Quantikine ELISA Kit, R&D Systems, Minneapolis, MN; Catalog Nos: Adiponectin: DY1065-05, PAI-1: DY1786, Hyaluronan: DY3614-05, Syndecan-1: DY2780, Glypican-1: DY4519-05) according to the manufacturer’s instructions.

Techniques: Sampling

Journal: Shock (Augusta, Ga.)

Article Title: Soluble T-Cadherin: A Novel Biomarker for Fluid Resuscitation in Burn Patients—A Single-Center Retrospective Observational Study

doi: 10.1097/SHK.0000000000002728

Figure Lengend Snippet: Correlation matrix between soluble T-cadherin and clinical/biochemical parameters. The size of each circle represents the absolute value of the correlation coefficient. The color scale ranges from blue (negative correlation) to red (positive correlation). Asterisks (*) indicate statistically significant correlations ( P < 0.05).

Article Snippet: Adiponectin and endothelial damage markers were measured using ELISA kits (Human T-Cadherin Quantikine ELISA Kit, R&D Systems, Minneapolis, MN; Catalog Nos: Adiponectin: DY1065-05, PAI-1: DY1786, Hyaluronan: DY3614-05, Syndecan-1: DY2780, Glypican-1: DY4519-05) according to the manufacturer’s instructions.

Techniques:

Journal: Brain, Behavior, & Immunity - Health

Article Title: The association of maternal obesity and race with serum adipokines in pregnancy and postpartum: Implications for gestational weight gain and infant birth weight

doi: 10.1016/j.bbih.2020.100053

Figure Lengend Snippet: Adiponectin, Leptin, and LAR throughout pregnancy and into post-partum. A) Adiponectin levels in black (left) and white (right) women throughout pregnancy and into postpartum. Adiponectin levels decreased from early to late pregnancy (p ​< ​1∗10 −4 ). Higher Pre-pregnancy BMI category was associated with lower adiponectin (ps ​< ​0.02). Black women had lower adiponectin levels at all time points versus white women within each BMI category (ps ​< ​0.05). B) Serum leptin levels in black (left) and white (right) women throughout pregnancy and into postpartum. Leptin levels increased throughout pregnancy (p ​< ​0.02) and declined in postpartum across both races (p ​< ​1∗10 −3 ). Lower leptin levels were associated with lower BMI categories in a step-wise manner (p ​< ​0.02). C) LAR in black (left) and White (right) women throughout pregnancy and into postpartum. LAR demonstrated a significant interaction (p ​< ​2∗10 −3 ) between time, BMI category, and race. Black women with obesity showed higher LAR in mid-pregnancy compared to early pregnancy (p ​< ​0.02) with levels that remained elevated in late pregnancy (p ​= ​0.72) and decreased in postpartum (p ​< ​1∗10 −3 ). In white women LAR did not change across pregnancy (ps ​> ​0.02). PP: postpartum. LAR: Leptin to adiponectin ratio. BMI: Body mass index.

Article Snippet: Serum levels of adiponectin were measured by diluting samples 1:1000 and assaying in duplicate using human adiponectin kits from Meso Scale Discovery (1601 Research Blvd.

Techniques:

Journal: Brain, Behavior, & Immunity - Health

Article Title: The association of maternal obesity and race with serum adipokines in pregnancy and postpartum: Implications for gestational weight gain and infant birth weight

doi: 10.1016/j.bbih.2020.100053

Figure Lengend Snippet: The Association of serum adiponectin, leptin, and LAR with Gestational Weight Gain.

Article Snippet: Serum levels of adiponectin were measured by diluting samples 1:1000 and assaying in duplicate using human adiponectin kits from Meso Scale Discovery (1601 Research Blvd.

Techniques:

Journal: Brain, Behavior, & Immunity - Health

Article Title: The association of maternal obesity and race with serum adipokines in pregnancy and postpartum: Implications for gestational weight gain and infant birth weight

doi: 10.1016/j.bbih.2020.100053

Figure Lengend Snippet: The association of serum adiponectin, leptin, and LAR with Infant Birth Weight.

Article Snippet: Serum levels of adiponectin were measured by diluting samples 1:1000 and assaying in duplicate using human adiponectin kits from Meso Scale Discovery (1601 Research Blvd.

Techniques: